CHAPTERS
Like many national associations, Phi Lambda Sigma operates through a system of local Chapters. Phi Lambda Sigma Chapters are designated by Greek letter. As of February 2019, a PLS Chapter had been chartered at 127 of the 142 schools and colleges of pharmacy across the United States.
| Region One | CT, MA, ME, NH, NY, RI, & VE |
| Region Two | DC, DE, MD, NJ, PA, VA, & WV |
| Region Three | AL, FL, GA, MS, NC, PR, SC, & TN |
| Region Four | IL, IN, KY, MI, OH, & WI |
| Region Five | IA, MN, NB, ND, SD, & WY |
| Region Six | AR, KS, LA, MO, OK, & TX |
| Region Seven | AK, ID, MT, OR, UT, & WA |
| Region Eight | AZ, CA, CO, HI, NM, & NV |